Genome-wide identification and characterization of long non-coding RNAs conferring resistance to Colletotrichum gloeosporioides in walnut (Juglans regia)

Abstract Background Walnut anthracnose caused by Colletotrichum gloeosporioides (Penz.) Penz. and Sacc. is an important walnut production problem in China. Although the long non-coding RNAs (lncRNAs) are for plant disease resistance, molecular mechanisms underlying resistance to C. remain poorly understood. Results The anthracnose-resistant F26 fruits from B26 clone anthracnose-susceptible F423 4–23 of were used as test materials. Specifically, we performed a comparative transcriptome analysis fruit bracts identify differentially expressed LncRNAs (DELs) at five time-points (tissues 0 hpi, pathological tissues 24 48 72 distal uninoculated 120 hpi). Compared with F423, total 14,525 DELs identified, including 10,645 upregulated lncRNAs 3846 downregulated F26. number compared was significantly higher early stages infection. A 5 modules related screened WGCNA target genes obtained. Bioinformatic showed that enriched immune-related processes during infection , such activation innate immune response, defense response bacterium, incompatible interaction system process, hormone signal transduction, phenylpropanoid biosynthesis other pathways. And 124 known 96 hub predicted, 10 genes. expression confirmed qPCR, which consistent RNA-seq data. Conclusions results this study provide basis future functional characterizations regarding bracts.